切换至 "中华医学电子期刊资源库"
高级检索
中华脑血管病杂志(电子版)

中华脑血管病杂志(电子版) ›› 2021, Vol. 15 ›› Issue (05) : 297 -301. doi: 10.11817/j.issn.1673-9248.2021.05.005

论著

大鼠脑缺血再灌注损伤后脑组织高迁移率族蛋白1表达的变化
许娜1, 渠静1, 王赛楠1, 江炜1, 耿晓坤1,†()   
  1. 1. 101149　首都医科大学附属北京潞河医院中美神经研究所
  • 收稿日期:2021-03-09 出版日期:2021-10-09
  • 通信作者: 耿晓坤

Expression of high mobility group protein box 1 at different time after cerebral ischemia-reperfusion

Na Xu1, Jing Qu1, Sainan Wang1, Wei Jiang1, Xiaokun Geng1()   

  1. 1. China-America Institute of Neuroscience, Beijing Luhe Hospital, Beijing 101149, China
  • Received:2021-03-09 Published:2021-10-09
  • Corresponding author: Xiaokun Geng
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

许娜, 渠静, 王赛楠, 江炜, 耿晓坤. 大鼠脑缺血再灌注损伤后脑组织高迁移率族蛋白1表达的变化[J]. 中华脑血管病杂志(电子版), 2021, 15(05): 297-301.

Na Xu, Jing Qu, Sainan Wang, Wei Jiang, Xiaokun Geng. Expression of high mobility group protein box 1 at different time after cerebral ischemia-reperfusion[J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2021, 15(05): 297-301.

目的

探讨大鼠脑缺血再灌注损伤后不同时间点脑组织高迁移率族蛋白1（HMGB1）表达的变化。

方法

40只雄性SD大鼠按随机数字表法分为4组，每组各10只。采用线栓法闭塞大鼠右侧大脑中动脉2 h诱导建立局灶性脑缺血再灌注损伤模型，分别于再灌后6 h、24 h、3 d及7 d后处死各组大鼠。TTC染色检测大鼠脑梗死体积，计算大鼠脑梗死体积比。蛋白质印迹法和ELISA法检测各组大鼠左、右侧脑组织HMGB1蛋白表达水平。RT-PCR检测各组大鼠左、右侧脑组织HMGB1 mRNA水平。

结果

蛋白质印迹法、ELISA法检测结果均显示，大鼠右侧脑组织HMGB1蛋白水平在脑缺血再灌注后6 h、24 h、3 d及7 d呈逐渐降低的趋势，左侧脑组织HMGB1蛋白水平也呈逐渐降低趋势。缺血再灌注后6 h大鼠右侧脑组织HMGB1蛋白水平高于左侧脑组织，且差异有统计学意义（P＜0.001），其他时间点大鼠左右侧脑组织HMGB1蛋白水平差异均无统计学意义（P均＞0.05）。RT-PCR检测结果显示，大鼠右侧脑组织HMGB1 mRNA水平在脑缺血再灌注后6 h、24 h、3 d及7 d呈逐渐降低的趋势，左侧脑组织HMGB1 mRNA水平呈先升高、后降低的趋势。缺血再灌注后6 h和24 h大鼠右侧脑组织HMGB1 mRNA水平均较左侧脑组织升高，且差异均有统计学意义（P均＜0.001），而其他时间点大鼠左右侧脑组织HMGB1 mRNA水平差异均无统计学意义（P均＞0.05）。

结论

HMGB1表达水平在脑缺血再灌注后6 h至7 d呈降低趋势。

关键词: 脑缺血再灌注, 高迁移率族蛋白1, 大脑
Objective

To study the expression of high mobility group protein box 1 (HMGB1) at different times in cerebral ischemia-reperfusion model of rats.

Methods

Forty male SD rats were divided into 6 h group, 24 h group, 3 d group and 7 d group by random number table method. The rat model of focal cerebral ischemia-reperfusion injury was established by occlusion of the middle cerebral artery with thread occlusion for 2 h. The volume of cerebral infarction was measured by TTC staining. The mRNA level of HMGB1 in rat brain tissue was measured by semi-quantitative RT-PCR, and the protein expression of HMGB1 was detected by ELISA and Western blotting at different times after reperfusion in rat middle cerebral artery acclusion (MCAO) model.

Results

The mRNA level and protein level of HMGB1 decreased at 6 h-7 d after ischemia-reperfusion. The mRNA level of HMGB1 in the right brain tissue was higher than that in the left brain tissue at 6 h and 24 h after cerebral ischemia reperfusion. The protein level of HMGB1 in the right brain tissue was higher than that in the left brain tissue 6 h after cerebral ischemia reperfusion.

Conclusion

The level of HMGB1 decreased at 6 h-7 d after cerebral ischemia-reperfusion.

Key words: Cerebral ischemia-reperfusion, High mobility group protein box 1, Cerebrum
图1 各组小鼠脑组织的TTC染色
表1 各组大鼠脑梗死体积比比较（n=5，%，
xˉ
±s）
组别 脑梗死体积比
再灌注6 h 11.4±4.1
再灌注24 h 35.2±4.7abc
再灌注3 d 20.4±1.6
再灌注7 d 12.7±6.8
图2 Western印迹法检测各组大鼠脑组织HMGB1蛋白水平结果
表2 各组大鼠脑组织HMGB1蛋白水平比较（n=5，
xˉ
±s）
组别 Western印迹法 ELISA法
右侧脑组织 左侧脑组织 右侧脑组织 左侧脑组织
再灌注6 h 240.6±8.8a 167.4±7.3 2 558.0±163.4a 1 678.0±177.0
再灌注24 h 156.8±13.0 150.0±16.6 1 676.0±122.2 1 711.0±161.8
再灌注3 d 122.4±11.9 118.2±11.1 1 476.0±173.5 1 648.0±150.8
再灌注7 d 103.6±8.8 101.2±7.5 1 668.0±86.2 1 650.0±55.0
表3 各组大鼠脑组织HMGB1 mRNA水平比较（n=5，
xˉ
±s）
组别 右侧脑组织 左侧脑组织
再灌注6 h 1.637±0.052 a 1.147±0.038
再灌注24 h 1.486±0.015 a 1.315±0.037
再灌注3 d 1.291±0.054 1.226±0.035
再灌注7 d 0.934±0.031 1.045±0.057
1
Richards LG, Cramer SC. Advances in stroke: therapies targeting stroke recovery [J]. Stroke, 2021, 52(1): 348-350.
2
Anrather J, Iadecola C. Inflammation and stroke: an overview [J]. Neurotherapeutics, 2016, 13(4): 661-670.
3
Zhang SR, Phan TG, Sobey CG. Targeting the immune system for ischemic stroke [J]. Trends Pharmacol Sci, 2021, 42(2): 96-105.
4
Geng X, Wang Q, Lee H, et al. Remote ischemic postconditioning vs. physical exercise after stroke: an alternative rehabilitation strategy? [J]. Mol Neurobiol, 2021. Online ahead of print.
5
Geng X, Shen J, Li F, et al. Phosphoenolpyruvate carboxykinase (PCK) in the brain gluconeogenic pathway contributes to oxidative and lactic injury after stroke [J]. Mol Neurobiol, 2021, 58(5): 2309-2321.
6
Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20(1): 84-91.
7
Xue J, Suarez JS, Minaai M, et al. HMGB1 as a therapeutic target in disease [J]. J Cell Physiol, 2021, 236(5): 3406-3419.
8
Wang C, Jiang J, Zhang X, et al. Inhibiting HMGB1 reduces cerebral ischemia reperfusion injury in diabetic mice [J]. Inflammation, 2016, 39(6): 1862-1870.
9
Ye Y, Zeng Z, Jin T, et al. The Role of high mobility group box 1 in ischemic stroke [J]. Front Cell Neurosci, 2019, 13: 127.
10
Zhang B, Shen J, Zhong Z, et al. PKM2 aggravates cerebral ischemia reperfusion-induced neuroinflammation via TLR4/MyD88/TRAF6 signaling pathway [J]. Neuroimmunomodulation, 2021, 28(1): 29-37.
11
Hatayama K, Chen RH, Hanson J, et al. High-mobility group box-1 and inter-alpha inhibitor proteins: In vitro binding and co-localization in cerebral cortex after hypoxic-ischemic injury [J]. FASEB J, 2021, 35(3): e21399.
12
Martinotti S, Patrone M, Ranzato E. Emerging roles for HMGB1 protein in immunity, inflammation, and cancer [J]. Immunotargets Ther, 2015, 4: 101-109.
13
Yanai H, Ban T, Taniguchi T. High-mobility group box family of proteins: Ligand and sensor for innate immunity [J]. Trends Immunol, 2012, 33(12): 633-640.
14
Gou X, Ying J, Yue Y, et al. The roles of high mobility group box 1 in cerebral ischemic injury [J]. Front Cell Neurosci, 2020, 14: 600280.
15
Wang Q, Zhao H, Gao Y, et al. Uric acid inhibits HMGB1-TLR4-NF-kappa B signaling to alleviate oxygen-glucose deprivation/reoxygenation injury of microglia [J]. Biochem Biophys Res Commun, 2021, 540: 22-28.
16
Lin CH, Chen HY, Wei KC. Role of HMGB1/TLR4 axis in ischemia/reperfusion-impaired extracellular glutamate clearance in primary astrocytes [J]. Cells, 2020, 9(12): 2585.
17
Wang J, Jiang Y, Zeng D, et al. Prognostic value of plasma HMGB1 in ischemic stroke patients with cerebral ischemia-reperfusion injury after intravenous thrombolysis [J]. J Stroke Cerebrovasc Dis, 2020, 29(9): 105055.
18
Watanabe H, Son M. The immune tolerance role of the HMGB1-RAGE axis [J]. Cells, 2021, 10(3): 564.
19
Wang Y, Li L, Deng S, et al. Ursolic acid ameliorates inflammation in cerebral ischemia and reperfusion injury possibly via high mobility group box 1/toll-like receptor 4/NF κB pathway [J]. Front Neurol, 2018, 9: 253.
20
Wang C, Jiang J, Zhang X, et al. Inhibiting HMGB1 reduces cerebral ischemia reperfusion injury in diabetic mice [J]. Inflammation, 2016, 39(6): 1862-1870.
21
Xiong X, Gu L, Wang Y, et al. Glycyrrhizin protects against focal cerebral ischemia via inhibition of T cell activity and HMGB1-mediated mechanisms [J]. J Neuroinflammation, 2016, 13(1): 241.
[1] 魏淑婕, 惠品晶, 丁亚芳, 张白, 颜燕红, 周鹏, 黄亚波. 单侧颈内动脉闭塞患者行颞浅动脉-大脑中动脉搭桥术的脑血流动力学评估[J]. 中华医学超声杂志（电子版）, 2023, 20(10): 1046-1055.
[2] 袁泽, 庄丽. 超声检测胎儿脐动脉和大脑中动脉血流对胎儿宫内窘迫的诊断价值[J]. 中华医学超声杂志（电子版）, 2023, 20(06): 618-621.
[3] 杨忠, 邓学东, 姜纬, 殷林亮, 潘琦, 梁泓, 马建芳, 苟中山, 王珍琦. 三维能量多普勒及彩色多普勒超声评估先天性心脏病胎儿脑血流灌注的研究[J]. 中华医学超声杂志（电子版）, 2020, 17(09): 874-879.
[4] 张海燕, 俞兆儿, 王一枭, 高明明, 贾瑞喆. 胎儿脐动脉舒张末期血流缺失及脑-胎盘率降低与妊娠结局及存活儿早期预后[J]. 中华妇幼临床医学杂志(电子版), 2020, 16(06): 665-671.
[5] 张玉凤, 万涵, 樊华峰, 袁娟, 刘瑞清, 徐鹏飞, 王军, 邓慧玲. 高迁移率族蛋白1在肠道病毒71型手足口病患者中的表达及临床价值[J]. 中华实验和临床感染病杂志(电子版), 2022, 16(01): 9-15.
[6] 关明函, 薛志强. 右美托咪定改善大鼠脑缺血再灌注后脑损伤的研究[J]. 中华神经创伤外科电子杂志, 2023, 09(05): 270-276.
[7] 李晓东, 魏云. 冠状切口额下入路治疗前颅窝巨大脑膜瘤[J]. 中华神经创伤外科电子杂志, 2023, 09(05): 318-319.
[8] 黎鹏程, 黄谦亦, 张逵, 范润金, 尚彬. 手术切除颅内巨大假性动脉瘤一例报道并文献复习[J]. 中华神经创伤外科电子杂志, 2023, 09(02): 123-125.
[9] 杨梦琦, 马慧芬, 訾阳, 王楠, 杜冰玉, 常万鹏, 于少泓. 马黛茶对脑血管疾病防治作用的研究进展[J]. 中华脑科疾病与康复杂志(电子版), 2023, 13(04): 235-240.
[10] 郑薏, 彭雯雯, 钟月丽. MicroRNA-34a调控电针对缺血再灌注损伤大鼠反应性星形胶质细胞的影响[J]. 中华脑科疾病与康复杂志(电子版), 2023, 13(03): 135-141.
[11] 胡飞龙, 路璐. 胞磷胆碱对大脑中动脉缺血性脑卒中恢复期的疗效[J]. 中华脑科疾病与康复杂志(电子版), 2023, 13(01): 51-56.
[12] 康晓宇, 刘丽旭. 心肺复苏后全脑缺血再灌注损伤的研究进展[J]. 中华脑科疾病与康复杂志(电子版), 2021, 11(02): 116-120.
[13] 江哲宇, 蒋天鹏, 周石, 王黎洲. 微小RNA在脑缺血再灌注损伤中的研究现状与进展[J]. 中华介入放射学电子杂志, 2022, 10(01): 75-82.
[14] 李永凯, 吐尔洪·吐尔逊, 杨建中. 开颅术后患者大脑中动脉的经颅多普勒超声测量值与意识评分、血生化指标的相关性[J]. 中华脑血管病杂志(电子版), 2023, 17(03): 221-226.
[15] 孙宁, 赵岩, 杨新宇. 颞浅动脉-大脑中动脉的直接搭桥术治疗低龄（5~11岁）儿童烟雾病11例[J]. 中华脑血管病杂志(电子版), 2021, 15(04): 242-246.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号