数字减影血管造影（DSA）引导下建立食蟹猴脑缺血再灌注（CIR）模型，探讨并评估多模态磁共振（MRI）成像技术在模型建立过程中的应用价值。

选取8只成年食蟹猴，术前排除脑血管畸形及颅内占位性病变，DSA引导下成功栓塞食蟹猴大脑中动脉（MCA），建立CIR模型。术后1 h行颅脑多模态MRI扫描确认栓塞位置及脑缺血范围，并于术后2.5 h行阿替普酶（rt-PA）静脉溶栓治疗，分别后于术后4 h、24 h及7 d再次行颅脑多模态MRI扫描明确治疗后影像学变化。术后第8天处死动物取脑组织进行2,3,5-三苯基氯化四氮唑（TTC）染色。

6只实验动物DSA下均证实栓塞成功。静脉溶栓再灌注治疗后4 h、24 h及7 d后弥散加权成像（DWI）异常高信号区相对面积逐渐减小，表观弥散系数（ADC）值持续升高，磁共振血管成像（MRA）显示MCA血流再通，血管信号强度逐渐增强，远端分支显影逐渐增多。脑组织TTC失染区相对面积较术后4 h DWI图像明显减小，与术后7 d MRI结果相符。

DSA引导下可成功建立猴脑CIR模型。多模态MRI的DWI、ADC及MRA成像技术可评价CIR模型血管再通情况及脑缺血面积变化。

To establish a cerebral ischemia-reperfusion (CIR) model in cynomolgus monkeys under digital subtraction angiography (DSA) guidance and evaluate the utility of multimodal magnetic resonance imaging (MRI) in the modeling process.

Eight adult cynomolgus monkeys were selected, with cerebrovascular malformations and intracranial space-occupying lesions excluded preoperatively. The middle cerebral artery (MCA) was successfully embolized under DSA guidance to establish the CIR model. Multimodal MRI was performed at 1 h post-operation to confirm the embolization site and ischemic area. Intravenous thrombolysis with recombinant tissue plasminogen activator (rt-PA) was administered at 2.5 h, followed by serial multimodal MRI scans at 4 h, 24 h, and 7 d post-operation to assess therapeutic changes. Animals were sacrificed on day 8 for 2,3,5-triphenyltetrazolium chloride (TTC) staining of brain tissues.

Successful embolization was angiographically confirmed in six animals. Following thrombolytic reperfusion, the relative area of hyperintensity on diffusion-weighted imaging (DWI) progressively decreased at 4 h, 24 h, and 7 d, accompanied by continuously increasing apparent diffusion coefficient (ADC) values. Magnetic resonance angiography (MRA) demonstrated MCA recanalization with gradual enhancement of vascular signal intensity and increased visualization of distal branches. The relative area of TTC-unstained regions was significantly reduced compared to 4 h DWI findings, correlating with 7 d MRI results.

Under DSA guidance, a cerebral ischemia-reperfusion (CIR) model in monkeys was successfully established. Multimodal MRI techniques, including diffusion-weighted imaging (DWI), apparent diffusion coefficient (ADC) mapping, and magnetic resonance angiography (MRA), could effectively evaluate vascular recanalization status and dynamic changes in cerebral ischemic area in the CIR model.