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中华脑血管病杂志(电子版) ›› 2023, Vol. 17 ›› Issue (02) : 136 -144. doi: 10.11817/j.issn.1673-9248.2023.02.009

循证医学

丹参酮ⅡA治疗急性缺血性脑卒中动物模型效果的Meta分析
邱佳敏1, 蒋惠怡2, 陶涛2,()   
  1. 1. 524023 广东湛江,广东医科大学第一临床医学院;524033 广东湛江,广东省湛江中心人民医院麻醉科
    2. 524033 广东湛江,广东省湛江中心人民医院麻醉科
  • 收稿日期:2022-03-29 出版日期:2023-04-01
  • 通信作者: 陶涛
  • 基金资助:
    广东省自然科学基金面上项目(2021A1515010897)

Efficacy of Tanshinone Ⅱ A on animal models of acute ischemic stroke:a meta-analysis

Jiamin Qiu1, Huiyi Jiang2, Tao Tao2,()   

  1. 1. The First School of Clinical Medicine, Guangdong Medical University, Zhanjiang 524023, China; Department of Anesthesiology, Central People's Hospital of Zhanjiang, Zhanjiang 524033, China
    2. Department of Anesthesiology, Central People's Hospital of Zhanjiang, Zhanjiang 524033, China
  • Received:2022-03-29 Published:2023-04-01
  • Corresponding author: Tao Tao
引用本文:

邱佳敏, 蒋惠怡, 陶涛. 丹参酮ⅡA治疗急性缺血性脑卒中动物模型效果的Meta分析[J]. 中华脑血管病杂志(电子版), 2023, 17(02): 136-144.

Jiamin Qiu, Huiyi Jiang, Tao Tao. Efficacy of Tanshinone Ⅱ A on animal models of acute ischemic stroke:a meta-analysis[J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2023, 17(02): 136-144.

目的

系统评价并分析丹参酮ⅡA对急性缺血性脑卒中动物模型的疗效。

方法

计算机检索Cochrane Library、Embase、Web of Science、Medline、中国知网、万方数据库和维普数据库,纳入有关丹参酮ⅡA对于急性缺血性脑卒中动物模型治疗的相关文献,检索年限为2003年2月至2021年2月。根据卒中治疗学术产业圆桌会议(STAIR)对动物实验研究进行质量评估。采用Review Manager 5.4对所纳入研究进行Meta分析。

结果

最终纳入14篇动物研究,共计218只动物,其中丹参酮ⅡA给药组为114只,对照组(赋形剂对照组)为104只。Meta分析结果显示,丹参酮ⅡA组与对照组相比,急性缺血性脑卒中动物给药后脑梗死体积(SMD=-3.12,95%CI:-3.59~-2.65)与神经功能评分(SMD=-1.87,95%CI:-2.35~-1.39)均存在明显下降,差异有统计学意义(P<0.0001)。

结论

丹参酮ⅡA对急性缺血性脑卒中的动物模型具有较好的治疗作用,并且展现了良好的神经保护作用,是一种相对安全的中药制剂。

Objective

To systematically evaluate and analyze the efficacy of Tanshinone ⅡA on animal models of acute ischemic stroke.

Methods

The Cochrane Library, Embase, Web of Science, Medline databases, CNKI, Wanfang, VIP database were searched by the internet. Literature on the treatment of Tanshinone ⅡA in animal models of acute ischemic stroke was included from February 2003 to February 2021. Quality assessments were conducted for experimental animal studies according to STAIR (stroke therapy academic industry roundtable). Meta-analysis was performed through Revman 5.4 software.

Results

14 animal studies were finally included, involving a total of 218 animals, of which 114 were administered Tanshinone ⅡA, and the other 104 were in the control group (excipients control group). Meta-analysis showed a significant decrease in cerebral infarction volume (SMD=-3.12, 95% CI: -3.59--2.65) and neuroscore (SMD=-1.87, 95% CI: -2.35--1.39) in the acute ischemic stroke group compared with the control group with statistically significant difference (P<0.0001).

Conclusion

Tanshinone ⅡA has a good therapeutic effect on animal models of acute ischemic stroke, and has shown a good neuroprotective effect, which means Tanshinone ⅡA is a relatively safe traditional Chinese medicine preparation.

图1 文献筛选流程图
表1 纳入研究的文献的基本特征
纳入研究 干预措施 动物模型(实验组/对照组,只) 卒中模型 给药方式 结局指标 质量评价[质量级别(满足条目),类(个)]
Cai M 2017[16] 丹参酮ⅡA 雄性 Nrf2 敲除小鼠(Nrf2 KO)(7/7) 短暂性大脑中动脉栓塞(1 h) 再灌注后10 min给药,腹腔注射,25 mg/kg 神经功能评分、脑梗死体积 Ⅰ(9)
Chen Y 2012[17] 丹参酮ⅡA 雄性SD大鼠(6/6) 短暂性大脑中动脉栓塞(2 h) 再灌注后10 min给药,腹腔注射,25 mg/kg 神经功能评分、脑梗死体积 Ⅱ(7)
Chen Y 2012[18] 丹参酮ⅡA 雄性SD大鼠(6/6) 短暂性大脑中动脉栓塞(2 h) 再灌注后10 min给药,腹腔注射,25 mg/kg 神经功能评分、脑梗死体积 Ⅰ(10)
Dong K 2009[19] 丹参酮ⅡA SD小鼠(9/3) 永久性大脑中动脉栓塞 造模前24 h和30 min给予腹腔注射20 mg/kg,造模后4 h重复腹腔注射20 mg/kg 神经功能评分、脑梗死体积 Ⅰ(9)
Liu L 2010[20] 丹参酮ⅡA 雄性SD大鼠(23/23) 右侧大脑中动脉栓塞 缺血发作后15 min腹腔注射给药20 mg/kg 神经功能评分、脑梗死体积 Ⅰ(9)
Li YH 2015[21] 丹参酮ⅡA 雄性Wistar大鼠(8/8) 短暂性左侧大脑中动脉栓塞(90 min) 再灌注后静脉注射给药40 mg/kg 神经功能评分、脑梗死体积 Ⅱ(5)
Tang C 2010[10] 丹参酮ⅡA SD大鼠(6/6) 短暂性大脑中动脉栓塞(2 h) 手术后5 min腹腔注射给药30 mg/kg 脑梗死体积 Ⅱ(5)
Tang Q 2012[22] 丹参酮ⅡA 雄性SD大鼠(4/4) 永久性大脑中动脉栓塞 胃内给药30 mg/(kg·d),1次/d,持续5 d即术前3 d,术后4 h和24 h 神经功能评分、脑梗死体积 Ⅰ(9)
Tang Q 2014[23] 丹参酮ⅡA 雄性SD大鼠(8/8) 颈总动脉结扎法 再灌注后1、4、6、12 h腹腔内给药25 mg/kg,1次/d,连续3 d 神经功能评分、脑梗死体积 Ⅰ(8)
Wang J 2020[24] 丹参酮ⅡA SD大鼠(5/5) 永久性大脑中动脉栓塞 手术后15 min腹腔注射给药20 mg/kg,1次/d,连续7 d 神经功能评分、脑梗死体积 Ⅰ(10)
Wang L 2010[25] 丹参酮ⅡA 雄性SD大鼠(6/6) 永久性大脑中动脉栓塞 造模后立即腹腔注射给药20 mg/kg 神经功能评分、脑梗死体积 Ⅰ(9)
Wen PY 2016[26] 丹参酮ⅡA 雄性SD大鼠(8/4) 短暂性大脑中动脉栓塞(2 h) 造模后腹腔注射8 mg/(kg·d),连续给药15 d 神经功能评分、脑梗死体积 Ⅰ(10)
Yang L 2011[27] 丹参酮ⅡA 雄性Wistar大鼠(10/10) 短暂性大脑中动脉栓塞(2 h) 胃内给药20 mg/(kg·d),连续7 d 脑梗死体积 Ⅱ(7)
叶龙彬 2004[28] 丹参酮ⅡA SD大鼠(8/8) 短暂性大脑中动脉栓塞(2 h) 手术前连续灌胃给药3 d,1次/d,25 mg/kg 神经功能评分、脑梗死体积 Ⅱ(6)
图2 丹参酮ⅡA治疗缺血性脑卒中动物模型的脑梗死体积森林图
图3 丹参酮ⅡA不同给药方式治疗缺血性脑卒中动物模型的脑梗死体积亚组分析森林图
图4 丹参酮ⅡA治疗缺血性脑卒中动物模型的神经功能评分森林图
图5 缺血性脑卒中动物模型脑梗死体积的漏斗图
图6 缺血性脑卒中动物模型神经功能评分漏斗图
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