Advances in probes and technologies for detecting cerebral ischemia-reperfusion injury

doi:10.3877/cma.j.issn.1673-9248.2025.02.010

Abstract

Abstract:

Ischemic stroke is a major global health challenge with profound impacts on human well-being.Reperfusion therapy represents the most effective intervention, yet successful blood flow restoration often triggers cerebral ischemia-reperfusion injury (CIRI), a complex pathological cascade that exacerbates structural damage and functional deficits.Real-time monitoring, early warning, and timely intervention are prerequisites for mitigating CIRI.Consequently, the development of highly sensitive and specific probes and technologies for CIRI detection has emerged as a research priority.This review systematically summarizes advances in molecular probes and analytical platforms targeting CIRI-related reactive species, mitochondrial dysfunction, and biothiol homeostasis.These innovations not only provide diagnostic and therapeutic insights for CIRI but also serve as critical tools for advancing neuroprotective strategies.

Key words: Stroke, Cerebral ischemia-reperfusion injury, Molecular probes, Oxidative stress, Biothiols

Qidan Pang, Wei Cui, Tao Tang, Dechun Jiang, Shen Li. Advances in probes and technologies for detecting cerebral ischemia-reperfusion injury[J]. Chinese Journal of Cerebrovascular Diseases(Electronic Edition), 2025, 19(02): 149-154.

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检测对象	应用目的	优势	局限性	特异性	应用模型
活性物质	活性分子的实时监测	灵敏度高、合成简洁	活性物质半衰期短，对探针灵敏度要求较高	高	细胞OGD/R、啮齿类MCAO
线粒体状态	线粒体功能状态监测	显示线粒体内环境状态	探针本身可能会影响线粒体的功能	高	细胞OGD/R、啮齿类MCAO
生物硫醇	硫醇类物质的实时监测	灵敏度高、合成简单	对硫醇类物质的差异性识别能力低	低	细胞OGD/R、啮齿类MCAO
其他	拉曼光谱机器学习分析	精确度高、减少人为的判断干扰	技术要求复杂、成本高	不明确	啮齿类MCAO

检测对象	应用目的	优势	局限性	特异性	应用模型
活性物质	活性分子的实时监测	灵敏度高、合成简洁	活性物质半衰期短，对探针灵敏度要求较高	高	细胞OGD/R、啮齿类MCAO
线粒体状态	线粒体功能状态监测	显示线粒体内环境状态	探针本身可能会影响线粒体的功能	高	细胞OGD/R、啮齿类MCAO
生物硫醇	硫醇类物质的实时监测	灵敏度高、合成简单	对硫醇类物质的差异性识别能力低	低	细胞OGD/R、啮齿类MCAO
其他	拉曼光谱机器学习分析	精确度高、减少人为的判断干扰	技术要求复杂、成本高	不明确	啮齿类MCAO

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